Single Cell Dna Amplification Kit

The genomeplex single cell whole genome amplification kit wga4 is designed to amplify the genome of a single cell resulting in a million fold amplification yielding microgram quantities of genomic dna.

Single cell dna amplification kit.

The genomeplex single cell wga kit wga4 containing the wga dna polymerase accurately amplifies plant dna as shown in the two model systems arabidopsis and tobacco. The kit includes all reagents required for cell lysis whole genome amplification enzymatic dna fragmentation and pcr free ngs library preparation. The qiaseq fx single cell dna library kit provides a complete solution for whole genome sequencing from isolated single animal or bacterial cells or low amounts of genomic dna. Single cell genomiphi dna amplification kit has been optimized to wholly amplify genomic dna from as little as a single cell generating micrograms of high quality dna for use in downstream applications.

In addition the mda reaction time has been shortened to 2 hours. Wga is achieved by pcr amplification of the library molecules using universal oligonucleotide primers. Traditional single cell whole genome amplification methods yield insufficient quantities with significant biased representation. Whole genome amplification from a single cell is now possible with our optimized genomeplex single cell whole genome amplification kit wga4.

Cdna libraries were amplified using the number of pcr cycles shown. The nebnext single cell low input cdna synthesis amplification module or the smart seq v4 ultra low input rna kit for sequencing clontech 634891 were used. Error bars indicate standard deviation for 8 21 replicates. At very low levels of input dna successful amplification relies on reagent quality.