Sirna Library Screening

The silencer select human apoptosis sirna library provides investigators with a focused sirna screening platform targeting the most up to date apoptosis gene targets thus enabling a detailed analysis of the apoptotic signaling pathway the silencer select human apoptosis sirna library contains three unique non overlapping sirnas per target for 924 genes.

Sirna library screening.

Library screening using genome wide sirnas as well as micrornas allows a researcher to analyze an entire signaling pathway of one or tens of thousands of genes. Prepare sirna liposome complexes in the wells of 96 well plates. A breast cancer cell line was transfected with a sirna library that targeted a variety of specific kinases. Fast and easy online configuration and ordering of plated sirna microrna reagents.

Take advantage of predefined gene families including druggable genes or whole genome. To better understand this complexity researchers are moving toward large screening projects to identify candidate genes involved in their biological question of interest. Adapted from hu et al. Freedom with custom library design.

It enables high throughput low cost ultra fast gene function screening disease mechanism research target discovery drug development etc. Sirna library provides a new and efficient way that bring about many original breakthroughs in biology especially for the discovery of new targets combined with. Rnai screening has allowed researchers to study the functionality of genes in a cell both individually and in. In the pursuit of gene functionality rnai library screening has become a crucial research tool used to study gene function and comprehensive signaling pathways.

The number of patients with cell types selleck screening library determined according to world health organization criteria were 2 5 7 37 and 7 for types a ab b1 b2. 1 transfection of cells with sirna pools is performed using reverse transfection. Silencer sirna is a great choice for economical screening of pre defined gene families and popular pathways like kinases cell surface receptors and dna damage response. Sirna library screening protocol.

Sirna library minimize time cost of screening using highly functional sirna. Sirna libraries composed of large numbers of sirnas have been developed and are used to identify sets of genes functionally involved in a particular biological process or disease. Screening often lies at the beginning of most discovery research. The sirna library brings a revolution in the efficiency of functional genomics research.

The network of possible molecular interactions leading to the causal phenotype within a given system pathway or disease is astounding. Genome wide sirna and microrna libraries. Cancer research 2012 14 r22.